A6001, A6002. $ 73. Add to Helix. Dec 12, 2023 · On Thursday November 9 th, 2023, Promega held its 7 th Biologics Symposium at the Babraham Research Campus in Cambridge. PCR Nucleotide Mix Protocol. The Maxwell® FSC DNA IQ™ Casework Kit is used with the Maxwell® FSC Instrument (Cat. In addition, the cells carry the F´ episome, which allows blue/white screening. We also offer XpressAmp™ Reagents that are compatible for use with the GoTaq® qPCR and RT-qPCR Systems and enable direct PCR amplification from viral samples without having to perform RNA or DNA extraction. Here you can find tools, resources, and support from real scientists to help your iGEM project succeed. The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene ( luc2 ). 6kDa) and Small BiT (SmBiT; 11 amino acids) subunits are fused to proteins of interest, and when expressed, the PPI brings the subunits into. PCR, the polymerase chain reaction, is a core technique that has revolutionized molecular biology. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 –20 moles of luciferase can be measured under optimal conditions. You are viewing: DD7101 Change Configuration. Luciferase reporter technology gives the researcher unparalleled sensitivity, dynamic range, versatility and ease of use when investigating questions that involve gene regulation. You can add notes and use timers during the protocol run, edit Promega Protocols to create your own custom protocols, or enter your own protocols and save them locally on your iOS device. We not only help you to scale the number of samples processed, but also the amount of biological material. PCR Nucleotide Mix is manufactured under cGMP conditions and has equimolar amounts of each dNTP to ensure optimal PCR. Reporter options include NanoLuc®, firefly and Renilla luciferases, which have different protein sizes. A C-terminal biotinylated human FcRn bound to. Panel A. Luminescence measured with the CellTiter-Glo® 2. Revenue is approaching $450 million (USD) in 2019. Your price: Log in. # AS4600) for optimal DNA extraction from forensic casework samples. Final Volume. Lumit™ HMGB1 Immunoassay reagents were added directly to the second plate of treated cells after 24 hours of treatment. Your price: Log in. Detect SARS-CoV-2 viral RNA in preprocessed wastewater with high efficiency and sensitivity using the SARS-CoV-2 RT-qPCR Kit for Wastewater. LDH is a widely used marker in. Note: Adjust total volume of diluted DNA for the appropriate number of wells being transfected. The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity. Finally this plasmid was linearized with EcoRI, mixed. These include reagents and kits for all steps in CE analysis workflows. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. Catalog number selected: E1330. Promega forensic products are manufactured in alignment with the ISO 18385 standard. Capable software engineers in the company were already fleeing by the time the ProMega was in ‘development’. Signal transduction cascades are generally triggered by the binding of ligands, such as growth factors, cytokines, neurotransmitters, or hormones, to a receptor. $ 244. Caspase-Glo® 3/7 Reagent was added directly to the cells in 96-well plates and incubated for 1 hour before recording luminescence. Identify unique alleles to resolve complex mixtures from related individuals or multiple sources. 20µg 5 × 20µg 1 × 100µg. Continue reading “Promega’s Top Three Most-Viewed Blog Posts of 2023”. The Lumit™ Detection Reagents A and B are specially designed to detect luminescence signal from Lumit™ Immunoassays when using labeled primary antibodies. Add to Cart. Working at Promega means growing and developing with a very innovative company that stands for progress and knows how to combine economic and social aspects. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be. When engaged with either an anti-TCR/CD3 stimulus alone or an anti-TCR/CD3 and an anti-CD28 stimulus, receptor-mediated signaling induces luminescence. Promega genomic DNA extraction products are designed for isolation of high-quality DNA from many sample types, including blood, cells, tissues, plants, microbes and more. The Maxwell® RSC Genomic DNA Kit used with the Maxwell® RSC or the Maxwell® RSC 48 Instrument consistently extracts high yield and quality of DNA from multiple sample types. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important. Tolerant To Trypsin-Inhibiting Contaminants. The one thing I’m not recollecting 100% is whether there were different versions of the app based on the bundled firmware. The small kit size (100 reactions) and low reaction volume (12. $ 91. Luciferin-IPA/10ml Assay. 6kDa) and Small BiT (SmBiT; 11 amino acids) subunits are fused to proteins of interest, and when expressed, the PPI brings the subunits into. The Promega Applications Guide is a comprehensive PDF document that provides useful information on various topics related to molecular biology, such as DNA and RNA purification, PCR, cloning, gene expression, cell culture, and more. The Caspase-Glo® 3/7 Assay is designed. Purity and yield of the extracted nucleic acids is key to performance in downstream applications such as PCR and sequencing. Complete listing of products for academic research, drug discovery, clinical research and diagnostics, applied sciences and human identification. Instructions for Use of Product (s) J2380, J2381. Founded in 1978, the company is headquartered in Madison. Software and Methods. See below for details of the vectors provided with each system. The Maxwell® Clinical Sample Concentrator (CSC) Instrument provides automated nucleic acid purification for a range of clinical sample types. You can add notes and use timers during the protocol run, edit Promega Protocols to create your own custom protocols, or enter your own protocols and save them locally on your iOS device. The Standard Warranty covers all parts and labor. The Spectrum Compact CE System is simple to set up and operate, with consumables like buffers and polymer prefilled in convenient packaging. GoTaq® DNA Polymerase is a proprietary formulation of Taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq. Dilute DNA by adding OptiMEM™ media and DNA to a microfuge tube. The small kit size (100 reactions) and low reaction volume (12. Your price: Log in. The GenePrint® 24 System is designed to generate a 5-dye 24-multilocus human DNA profile from a variety of human-derived biological sources. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8. Sensitive, Rapid and Convenient Dual-Reporter Assay. The assay consists of a genetically engineered Jurkat T cell line that expresses human OX40 and a. The assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51 Cr] is released in radioactive assays. pansy), represent a large part of the Promega luminescence portfolio. org December 21, 2023 The new england journal of medicine 2022 Investigation We interviewed Patient 2 in May and June 2022 and reinterviewed Patient 1 on June 27. Log in. The system is designed for use with a vacuum source and vacuum manifold, greatly reducing the time spent on purification. Promega real-time PCR (qPCR) and RT-qPCR assays provide a choice of probe-based or dye-based qPCR methods. Immunoassays are convenient and widely used detection methods based on the ability of antibodies to bind and detect specific antigens. , Ltd Global Trade Center, B907 - 909 36 Eastern Road, 3rd North Ring Road Dong Cheng District Beijing 100013 China Marketing Area: China. Addition of the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate and generation of a “glow-type” luminescent signal. Insert amount (ng) = Vector amount x Insert size / Vector size x (Molar ratio) Molar ratios (Insert/Vector ratio) of 3:1 are used for optimizing cohesive end ligations whilst higher molar ratios are used for blunt end ligations. In the DLR™ Assay, the activities of firefly ( Photinus pyralis) and Renilla ( Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The capillary array is positioned behind the oven door and lies flat against the oven, ensuring consistent capillary temperatures during electrophoresis. Nov 18, 2022 · He joined Promega in 2001 after retiring as Senior Vice President and Director of Anti-Infectives Drug Discovery and Development at GlaxoSmithKline. Cell health was also measured for each. The T Cell Activation Bioassay (IL-2), Propagation Model, is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used for the discovery and development of novel biologic and cell therapies aimed at inducing, strengthening and/or engineering T cell responses. The Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-Lactate in biological samples. The HiBiT Control Protein can be used as a positive control of known concentration when using the Nano-Glo® HiBiT Lytic Detection System (Cat. These assays are useful for interrogating a range of biological functions, including Fc effector activity, immune checkpoint modulation, T cell activation, and cytokine and growth factor signaling. At Promega Corporation, creativity and connection drive discovery and innovation. Addition of the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate and generation of a “glow-type” luminescent signal. Topics pertain to molecular and cellular biology, including nucleic acid purification, amplification, cloning, professional development, and more. Promega continues to expand our collection of HiBiT, NanoLuc®, and HaloTag® CRISPR knock-in cell pools and clones, including both N and C-terminal fusions to many popular drug targets. There are two ready-to-use. The Luciferase Assay System incorporates coenzyme A (CoA) for improved kinetics, allowing greater enzymatic turnover and resulting in increased light intensity that is nearly constant for at least 1 minute. The T Cell Activation Bioassay (IL-2), Propagation Model, is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used for the discovery and development of novel biologic and cell therapies aimed at inducing, strengthening and/or engineering T. The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. pmirGLO Dual-Luciferase miRNA Target Expression Vector. The mitochondrial calcium uniporter (MCU) plays essential roles in mitochondrial calcium homeostasis and regulates cellular functions, such as energy synthesis, cell growth, and development. Links to the entire contents of the Promega product catalog are accessible from this page. Add to Cart. Detecting and quantitating small amounts of RNA is important for many biological applications, including determining yield of in vitro transcribed RNA and measuring RNA concentration before Northern blot. The Maxwell® RSC Instrument can process from 1 to 16 samples and the Maxwell® RSC 48 can process from 1 to 48. The Caspase-Glo® 3/7 Reagent is optimized for caspase activity, luciferase activity and cell lysis. Bacterial Strain JM109. These samples include blood stains, semen stains, hair, cigarette butts, tissue samples and “touch” DNA samples regularly encountered in forensic DNA analysis. Angus. Promega was named a top employer in the US for a second year in a row, winning a 2023 Top Workplaces USA award. The Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-Lactate in biological samples. PCR, the polymerase chain reaction, is a core technique that has revolutionized molecular biology. Promega will break ground Thursday on a 150,000-square-foot manufacturing center. Maxwell® Instruments offer benchtop automation with worry-free, walk-away purification of DNA, RNA or total nucleic acid. The LigaFast™ System is designed to eliminate. 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised 8/23 www. ‘Any printers’ being the Pro and Micro/Micro+ yes. Obtain optimal heterozygote balance using up to 5ng of DNA template. When engaged with either an anti-TCR/CD3 stimulus alone or an anti-TCR/CD3 and an anti-CD28 stimulus, receptor-mediated signaling induces luminescence. Trypsin/Lys-C Mix, Mass Spec Grade. El sistema permitirá a los laboratorios forenses, clínicos y de investigación realizar secuenciación Sanger y análisis de fragmentos para distintas aplicaciones. 2017 (Post GST Implementation) A1541 Promega 100 Barrier Tips, 960/pk 10-100microl 1 12128 38220090 A1551 Promega 200 Barrier Tips, 960/pk 50-200microl 1 12128 38220090 A1563 Promega 1000 Barrier Tips, 768/pk100-1,000microl 1 12206 38220090 A1620 Wizard Genomic DNA Purification Kit 100. The CellTiter 96® AQ ueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. 69,561 followers. The Standard Warranty covers all parts and labor. 5X GoTaq® Flexi Reaction Buffers do not contain magnesium to allow for PCR optimization. pmirGLO Dual-Luciferase miRNA Target Expression Vector. Meet David Goodsell. Software and Methods. Literature # TB317. Wastewater Surveillance for Tracking COVID-19 and Other Diseases. $ 435. Yeast protein extract was used as a model system. T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. $ 435. The P450-Glo™ CYP3A4 Assays provide a homogeneous, luminescent method for measuring cytochrome P450 CYP3A4 activity. Your price: Log in. LDH is a widely used marker in. DNA Step Ladders have defined sizes with exact incremental steps between bands. 8 at 25°C), 100mM MgCl 2, 100mM DTT and 10mM ATP. The solution can be used to detect both double-stranded and single-stranded DNA. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. $ 84. HiBiT technology simplifies protein tagging in live cells, providing a streamlined protocol for detecting tagged proteins that requires only a luminometer for detection. Promega Corporation | 69,803 followers on LinkedIn. The OncoMate™ MSI Dx Analysis System is a fluorescent, multiplex PCR-based test to detect microsatellite instability (MSI) in colorectal cancer tumor samples. During culture,. $ 435. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment. $ 525. Interstate and outer-Sydney orders must be received by 3:00 pm on Tuesday, 19 December. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly (A)+ RNA. Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. Adding dNTPs as a mix also simplifies pipetting steps and reduces the risk of contamination. The final PCR product was inserted into pGEM-T Easy (Promega), generating plasmid pPfRh5_C-term_LoxP. Lactate dehydrogenase (LDH) is a soluble stable cytosolic enzyme present in many cell types that is rapidly released into the cell culture medium upon disruption of the plasma membrane. Polymer4 is optimized for fragment analysis, and. You can add notes and use timers during the protocol run, edit Promega Protocols to create your own custom protocols, or enter your own protocols and save them locally on your iOS device. OX40 Bioassay is a bioluminescent cell-based assay that measures the potency and stability of ligands or agonist antibodies that can bind and activate OX40. Two fluorescence channels for nucleic acid and protein quantitation. Promega offers an extensive toolbox of reporter bioassays to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. The company’s next generation targets, indexed to revenue over a 2019 baseline, include: Emissions: 50. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Catalog number selected: E1330. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. A Human IgG1 labeled with LgBiT (Tracer-LgBiT) is used as the tracer. Use version BOSSA 1. The Spectrum Compact CE System is an automated 4-capillary electrophoresis instrument designed for a wide range of sequencing and fragment analysis applications. The data show that digestion at low pH was comparable to digestion at conventional conditions. The PowerQuant® System is a 5-color, 4-target probe-based qPCR assay that quantifies the total amount of amplifiable autosomal and Y-chromosomal DNA in a single assay using the same DNA standards. Lab automation equipment includes the Maxprep™ Liquid Handler for sample preparation and the Maxwell® RSC. Promega Corporation was founded by Bill Linton in 1978 to provide restriction enzymes for biotechnology. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 –20 moles of luciferase can be measured under optimal conditions. This master mix does not contain a reference dye; however, a. Your price: Log in. Bioluminescence imaging was done at the University of Wisconsin Small Animal Imaging and Radiotherapy Facility. An Introduction to Transfection. 8 at 25°C), 100mM MgCl 2, 100mM DTT and 10mM ATP. Find out more about how. DNA-Dependent Protein Kinase (DNA-PK) phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T antigen and several transcription factors. The cultural diversity and scientific inspiration make the company a place where people enjoy working. OX40 Bioassay is a bioluminescent cell-based assay that measures the potency and stability of ligands or agonist antibodies that can bind and activate OX40. With the dynamic range to detect proteins in live cells without overexpression, and the convenience of a single-reagent-addition method, HiBiT technology opens up a universe of. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7. Add to Cart. , peptides with C-terminal arginine and lysine residues). Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be. Add to Cart. The Lumit™ Immunoassay Labeling Kit allows the users to label their antibodies and proteins with HaloTag®-SmBiT and HaloTag®-LgBiT to enable the development of a Lumit™ Immunoassay. The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. com 1. Trypsin Digestion in as Little as 60 Minutes. A collection of interactive web tools and downloadable software to help you do your work, including calculators for molecular biology research, primer designers, and instrument. Promega (Beijing) Biotech Co. Promega's Cookie Policy We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. An Introduction to Transfection. A6001, A6002. 0 Assay is proportional to the number of viable Jurkat cells in. Jurkat cells were treated with anti-Fas mAb for 4. Restriction Enzyme Tables. RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. Lumit™ HMGB1 Immunoassay reagents were added directly to the second plate of treated cells after 24 hours of treatment. Panel A. Over the past year, Promega has been recognized with additional. To apply PCR to the study of RNA, the RNA sample must first be reverse transcribed to cDNA to provide the necessary DNA template for subsequent PCR. The OX40 Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays. Introduction to RT-PCR. GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction. E2670, E2671. GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. GoScript uses M-MLV reverse transcriptase and state-of-the-art buffer to drive robust, reliable cDNA synthesis of a full range of rare and. Luciferase reporter technology gives the researcher unparalleled sensitivity, dynamic range, versatility and ease of use when investigating questions that involve gene regulation. Reporter options include NanoLuc®, firefly and Renilla luciferases, which have different protein sizes. The stringent specificity of trypsin is essential for protein identification, and it has become the gold standard for protein digestion to peptides for shotgun proteomics. The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Note: Adjust total volume of diluted DNA for the appropriate number of wells being transfected. Your price: Log in. Finally this plasmid was linearized with EcoRI, mixed. JM109 is a K strain that is rec A– and end A– to minimize recombination and improve the quality of plasmid DNA. The OX40 Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays. Downloadable software and firmware for Promega instruments, including luminometers, fluorometers and DNA purification instruments. This solution is ready to use and. Note: Adjust total volume of diluted DNA for the appropriate number of wells being transfected. Wastewater Surveillance for Tracking COVID-19 and Other Diseases. The OX40 Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays. These include trypsin options for rapid digestion, digestion of resistant proteins, immobilized. The Maxwell® RSC simplyRNA Cells Kit, used with the Maxwell® RSC Instruments, provides a simple method for automated RNA purification from fresh or frozen cells. Please Enquire. The master mix is composed of an optimized buffer formulation complete with dNTPs and. Promega genomic DNA extraction products are designed for isolation of high-quality DNA from many sample types, including blood, cells, tissues, plants, microbes and more. The new GoTaq® qPCR Master Mix is a 2X master mix for quantitative PCR (qPCR) and features GoTaq® Hot Start Polymerase, a full-length Taq DNA polymerase bound to a proprietary antibody that prevents polymerase activity at room temperature. Generally, 100-fold greater sensitivity can be achieved over. Promega was named a top employer in the US for a second year in a row, winning a 2023 Top Workplaces USA award. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. 0 Assay is proportional to the number of viable Jurkat cells in. Over the past year, Promega has been recognized with additional. Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3 and CD19 bispecific T cell engager). The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. The Maxwell® FSC DNA IQ™ Casework Kit is used with the Maxwell® FSC Instrument (Cat. Yeast protein extract was used as a model system. Add to Helix. Includes an overview of the methods and systems available for in vitro translation of proteins, including eukaryotic and prokaryotic-based cell free expression systems. An overview of products used for protein expression, purification and analysis. The Promega Protocols app allows you to select and run protocols for a range of Promega kits. Trypsin/Lys-C Mix, Mass Spec Grade. Both kits contain three components: i) protease (Trypsin or Trypsin/Lys-C Mix); ii) protease Resuspension Buffer; and iii) Rapid-Digestion Buffer. There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries: 1) disruption of the cellular structure to create a lysate, 2) separation of the soluble DNA from cell debris and other insoluble material, 3) binding the DNA of interest to a purification matrix, 4) washing proteins and other contaminants away from. Instructions for Use of Product(s) J1631. T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. The Maxwell® RSC Genomic DNA Kit used with the Maxwell® RSC or the Maxwell® RSC 48 Instrument consistently extracts high yield and quality of DNA from multiple sample types. PCR Master Mix is stable for 3 months when stored at 4°C. Panel A. 6kDa) and Small BiT (SmBiT; 11 amino acids) subunits are fused to proteins of interest, and when expressed, the PPI brings the subunits into. The assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51 Cr] is released in radioactive assays. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The 5X GoTaq® Green and Colorless Reaction Buffers supplied with GoTaq® DNA Polymerase contain MgCl 2 at a concentration of 7. Over the past year, Promega has been recognized with additional. Proteolysis with Trypsin/Lys-C Mix, Mass Spec Grade, is specific and generates tryptic peptides (i. The enzyme will not join single-stranded nucleic acids. These samples may include blood stains, semen stains, hairs, cigarette butts, tissue samples and trace DNA samples regularly encountered in forensic DNA analysis. We offer a full range of luciferase assay products and tools for your research. The EC 10 and EC 90 concentrations to. Instructions for Use of Product(s) J1631. Or you’re just trying to get a job. It is compatible with existing fluorescently-labeled dideoxynucleotide triphosphate-based chain termination sequencing chemistries along. Here is an example of measuring drug-induced toxicity in HCT116 spheroids. The development of reporter gene systems and selection. Now you can design the right assay in the right cells to model the biology you are studying. Adding dNTPs as a mix also simplifies pipetting steps and reduces the risk of contamination. Knock-ins are performed in common workhorse cell lines and, in many cases, the cell background that is most relevant to the biology being studied. Yeast protein extract was used as a model system. Luminescence measured with the CellTiter-Glo® 2. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly (A)+ RNA. The Lumit™. DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly (A)+ RNA. seductive tease
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Maxprep™ Liquid Handler Consumables. Lumit™ HMGB1 Immunoassay reagents were added directly to the second plate of treated cells after 24 hours of treatment. The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. The small kit size (100 reactions) and low reaction volume (12. Rapid hot-start activation and processive enzymes make the GoTaq® Systems compatible. Complete listing of products for academic research, drug discovery, clinical research and diagnostics, applied sciences and human identification. Literature # TB317. Includes an overview of the methods and systems available for in vitro translation of proteins, including eukaryotic and prokaryotic-based cell free expression systems. The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. He spent 20 years in the SmithKline R&D Organization and served as Senior Vice President and Director in R&D since 1993. Caroline End, Product Manager, Promega Germany. Our product portfolio of over 4,000 products supports a range of life science work in cell biology, DNA, RNA and protein analysis, drug development, human identification and molecular diagnostics. Human Female/100µg. The RealTime-Glo™ MT Cell Viability Assay generates a luminescent signal that is dependent upon continuous reduction of the MT cell viability substrate by viable cells and rapid turnover by NanoLuc® Luciferase. Calculate melting temperature of Oligonucleotides. The DNA fragments may be stained with ethidium bromide. Our product portfolio of over 4,000 products supports a range of life science work in cell biology, DNA, RNA and protein analysis, drug development, human identification and molecular diagnostics. In ammonium bicarbonate and ammonium acetate the enzyme specificity is higher at the glutamic residues. Add to Cart. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly (A)+ RNA. Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. Lactate is produced by glycolysis, a major metabolic pathway responsible for glucose homeostasis and energy production. The CellTiter-Glo® 2. The RealTime-Glo™ Annexin V Apoptosis and Necrosis assay is non-lytic and the simple “add-and-read” method allows multiple readings from a single assay well. The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. Improve productivity with rapid-cycling and more loci. a. 20µg 5 × 20µg 1 × 100µg. Products include preprocessing reagents for swabs and card punches prior to direct amplification of database samples, and DNA isolation systems for casework samples. 1kb DNA Step Ladder. The Spectrum Compact CE System offers Sanger sequencing and fragment analysis right on your benchtop. 25ml 50ml 150ml 500ml 1,000ml. GoTaq® qPCR Master Mix is provided as a simple-to-use, stabilized 2X master mix that includes all components for quantitative PCR except sample DNA, primers and water. The reagent is prepared by mixing Nano-Glo® Luciferase Assay Substrate and Nano-Glo® Luciferase Assay. Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. Over the past year, Promega has been recognized with additional. The Rapid-Digestion Trypsin and Rapid-Digestion Trypsin/Lys-C Kits are designed to shorten protein digestion times to 60 minutes versus the typical 4–18 hours required for trypsin digestion. The P450-Glo™ CYP3A4 Assays provide a homogeneous, luminescent method for measuring cytochrome P450 CYP3A4 activity. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 10 8. Promega Corporation was founded by Bill Linton in 1978 to provide restriction enzymes for biotechnology. GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction. Key components of a PCR amplification reaction are a thermostable DNA polymerase, Deoxynucleotide. RQ1 RNase-Free DNase. The assay measures the reducing potential of viable cells and is ATP. Note: Adjust total volume of diluted DNA for the appropriate number of wells being transfected. Links to the entire contents of the Promega. The one thing I’m not recollecting 100% is whether there were different versions of the app based on the bundled firmware. Over the past year, Promega has been recognized with additional. 5ml (100/pack) DNA IQ™ Spin Baskets (50/pack) Promega forensic products are manufactured in alignment with the ISO 18385 standard. T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Add to Helix. In the presence of NADH a pro. See below for details of the vectors provided with each system. Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation. PCR, the polymerase chain reaction, is a core technique that has revolutionized molecular biology. Because JM109 is rec A– and lacks the E. 0kb vector in a ligation. $ 525. Conclusions: Use of Trypsin/Lys-C. Compatibility with databases around the world allows for fast identity confirmation and matching probability calculation, improving labs overall. com PRODUCT USE LIMITATIONS, WARRANTY, DISCLAIMER Promega manufactures products for a number of intended uses. GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Each point represents the average of 4 wells. It can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate OX40. Optimized conditions enable amplification of as few as 2 copies of target template. A guide to cell-free protein expression systems. Your price: Log in. The Maxwell® RSC Genomic DNA Kit used with the Maxwell® RSC or the Maxwell® RSC 48 Instrument consistently extracts high yield and quality of DNA from multiple sample types. This solution is ready to use and. Meet David Goodsell. Human Genomic DNA is purified and stored in 10mM Tris-HCl (pH 8. Introduction to RT-PCR. The Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-Lactate in biological samples. The strain grows well and is transformed efficiently by a variety of methods. Your price: Log in. 08; SV vacuum method, 1. Instructions for Use of Product(s) J1631. NanoLuc® Binary Technology (NanoBiT™) is a two-subunit system based on NanoLuc® luciferase that can be used for intracellular detection of protein:protein interactions (PPIs). Luminescence measured with the CellTiter-Glo® 2. 69,561 followers. Promega product catalog supplement 2021–2022. Contact Customer Service to check pricing and availability. Renilla Luciferase Assay System is designed to provide a fast, sensitive method of detecting luciferase from sea pansy ( Renilla reniformis ). Complementary DNA (cDNA) was synthesized using 1 µg of total RNA, an oligo(dT)20 primer and the Superscript III reverse. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. For the first time, participants had the option to attend the event either in person or experience it via live stream, creating an inclusive and dynamic environment where the latest breakthroughs and ideas could be showcased. The RealTime-Glo™ Annexin V Apoptosis and Necrosis assay is non-lytic and the simple “add-and-read” method allows multiple readings from a single assay well. $ 84. Achieve better consistency in detecting low level mixtures. Cell health was also measured for each. Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Identify unique alleles to resolve complex mixtures from related individuals or multiple sources. Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. If you want to configure multiple Promegas with static IP addresses you will have to give the Promegas each a unique IP address that is free on your network with the M552 command. Req ID: 3667. You can add notes and use timers during the protocol run, edit Promega Protocols to create your own custom protocols, or enter your own protocols and save them locally on your iOS device. Identify unique alleles to resolve complex mixtures from related individuals or multiple sources. We offer a full range of luciferase assay products and tools for your research. Luminescence measured with the CellTiter-Glo® 2. Continue reading "Promega's Top Three Most-Viewed Blog Posts of 2023". This award recognizes Promega for not only offering great benefits, but for putting employees first, unlocking their potential and inspiring their growth and performance. Literature # 9PIC114. Catalog number selected: MC1191. The Spectrum Compact CE System is an automated 4-capillary electrophoresis instrument designed for a wide range of sequencing and fragment analysis applications. ‘Any printers’ being the Pro and Micro/Micro+ yes. [2] The company now offers more than 4,000 life science products used by scientists, researchers and life science and pharmaceutical companies. Purity and yield of the extracted nucleic acids is key to performance in downstream applications such as PCR and sequencing. In PCR, a DNA molecule is targeted and copied multiple times, allowing exponential amplification of the target DNA sequence. Add to Cart. Peek inside the mind of the scientist who created our amazing scientific paintings. One of the easiest methods for cloning blunt-ended DNA fragments including PCR products is T-vector cloning, such as with pGEM®-T or pGEM®-T Easy Vector Systems. , peptides with C-terminal arginine and lysine residues). N is the number of nucleotides and [Loading] 660 p g p m o l is the average molecular weight of a nucleotide pair. A page made up of webinars, articles, and videos designed to help young scientists better understand what they are doing inside and outside of the lab. It is compatible with existing fluorescently-labeled dideoxynucleotide triphosphate-based chain termination sequencing chemistries along. 1 each. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Instructions for Use of Product (s)CE1304. PCR, the polymerase chain reaction, is a core technique that has revolutionized molecular biology. The Maxwell® FSC DNA IQ™ Casework Kit is used with the Maxwell® FSC Instrument (Cat. Capable software engineers in the company were already fleeing by the time the ProMega was in ‘development’. Ethidium Bromide Solution, Molecular Grade. The master mix allows sensitive detection and quantification even in the presence of reverse transcriptase inhibitors or PCR inhibitors. Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. Detection limit is defined as greater than three standard deviations above background RFU. The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The Lumit™ IFN-γ (Human) Immunoassay reagents were added directly to treated cell wells to detect IFN-γ release from the T cells. PCR Nucleotide Mix is manufactured under cGMP conditions and has equimolar amounts of each dNTP to ensure optimal PCR. For the first time, participants had the option to attend the event either in person or experience it via live stream, creating an inclusive and dynamic environment where the latest breakthroughs and ideas could be showcased. 5 × 200μl. Promega offers an extensive toolbox of reporter bioassays to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. In phosphate buffers cleavage occurs at the aspartic and glutamic residues. (2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. . craigslist wasilla ak, slipthegame, florida keys jobs, vintage sheffield pocket knives, meteorologist joe bartosik, craigslist s florida, karely ruiz porn, r4 revolution for ds ndsl nds firmware download, black stockings porn, used teardrop trailer for sale near me, hairy pregnant pussy pictures, cara menghitung togel bagi pemula co8rr